Billy West
Dr. Hunk (voice) / Carlton (voice) / Log Cabin Tom (voice) / Anderson Pooper (vo...

Will Matthews
Rick Brocka Jr. (voice) / Timmy (voice) / Echinacea (voice) / F. Cornelious Hamp...

Alan Cumming
Chuck Masters (voice) / Zeke (voice) / Chuck (voice) / Rupert (voice)

Wilson Cruz
Evan Martinez (voice) / Charo (voice)

Taylor M. Dooley
Dana Bernstein (voice)

Liza del Mundo
Ebony / Arugula (voice) / Pussy / Ursula (voice) / Minda / Pussy / Ebony (voice)...

Margaret Cho
Condie Ling (voice) / Condie Ling / Jessica (voice) / Condie Ling / Kathy Griffi...

Emily Brooke Hands
Kirsten (voice) / Straight Woman / Protestor (voice) / Heather (voice) / Mrs. Pe...

Lori Alan
Dixie / Nerd (voice) / Dixie / Mail Woman / Eunice (voice) / Ivory / Dr. Joy / A...

Jessica-Snow Wilson
Kirsten (voice) / Kirsten / Mary Jane (voice)

Lorna Luft
Joanna (voice)

Alec Mapa
Uncle Bakla (voice)

Kimberley Locke
Steamroom Sally (voice)

RuPaul
Tyler (voice)

Laraine Newman
Chastity Bono / Announcer / Additional Voices (voice)

Mitch Morris
Preston Fessler (voice)

Mark Hamill
Saul (voice)

Perez Hilton
Self (voice)

Jim J. Bullock
Jacques-Jean / Emerald Joe (voice)

Jennifer Coolidge
Fannie Mae (voice)