Jason Marsden
Duke (voice)

Matthew Yang King
Tunnel Rat (voice)

Kevin Michael Richardson
Roadblock (voice)

Natalia Cigliuti
Scarlett (voice)

David Kaye
Main Title Narrator/Professor Patrick O'Hara

Tatyana Yassukovich
Baroness (voice) / Anastasia Cisarovna/Baroness/Receptionist (voice)

Charlie Adler
Cobra Commander (voice) / Adam DeCobray/Cobra Commander (voice) / Cobra Commande...

Charlie Schlatter
Dr. Mindbender (voice) / Dr. Brian Bender/Dr. Mindbender (voice) / Dr. Mindbende...

Johnny Messner
Flint (voice)

Nika Futterman
Lady Jaye (voice) / Nika Futterman Lady Jaye/Female Reporter (voice) / Lady Jay...

Khary Payton
Ripcord (voice) / Wallace Weems/Ripcord/Lead Trooper (voice)

Clancy Brown
Destro (voice) / James McCullen/Destro/Whistleblower (voice) / James McCullen/Tr...

Andrew Kishino
Storm Shadow (voice) / Storm Shadow/Lead Trooper/Custodian (voice) / Storm Shado...

Kim Mai Guest
Jinx (voice)

Lee Majors
General Abernathy (voice)

Brian Bloom
Zartan (voice) / Zartan/Inventor/Dispatcher Voice (voice)

Keone Young
Hard Master/Arata (voice)

Danny Cooksey
Young Snake Eyes (voice)

André Sogliuzzo
Sebastian Bludd/Major Bludd/Store Manager (voice) / Major Bludd (voice)

Stephen Stanton
Tomax/Xamot (voice)