Seth Green
The Nerd / Batman / Robin / Aquaman / Additional Voices (voice)

Breckin Meyer
Superman / Plastic Man (voice)

Alex Borstein
Wonder Woman (voice)

Nathan Fillion
Green Lantern (voice)

Matthew Senreich
The Flash / Brainiac (voice)

Jonathan Banks
Composite Superman (voice)

Alfred Molina
Lex Luthor (voice)

Giovanni Ribisi
The Joker / Two-Face (voice)

Paul Reubens
The Riddler (voice)

Adam West
60's Batman / Bank Robber (voice)

Burt Ward
Self (voice)

Hugh Davidson
Martian Manhunter / Tran-Zar (voice)

Dee Bradley Baker
Ra's al Ghul (voice)

Kevin Shinick
Narrator (voice)

Mae Whitman
Power Girl (voice)

'Weird Al' Yankovic
Rubberduck (voice)

Shooter Jennings
Mr. Banjo (singing voice)