Richard Greene
Sir Henry Baskerville

Basil Rathbone
Sherlock Holmes

Wendy Barrie
Beryl Stapleton

Nigel Bruce
Doctor John H. Watson

Lionel Atwill
Dr. James Mortimer

John Carradine
Barryman

Barlowe Borland
Frankland

Beryl Mercer
Mrs. Jennifer Mortimer

Morton Lowry
John 'Jack' Stapleton

Ralph Forbes
Sir Hugo Baskerville

E. E. Clive
London Cabbie John Clayton

Eily Malyon
Mrs. Barryman

Lionel Pape
The Coroner

Nigel De Brulier
The Notting Hill Murderer

Mary Gordon
Mrs. Hudson

Ian Maclaren
Sir Charles Baskerville

Ruth Terry
Betsy Ann

Mary Young
Betsy Ann's Mother (uncredited)

Kenneth Hunter
Ship's Officer (uncredited)

Vesey O'Davoren
Ship's Steward (uncredited)