Andrew Rannells
Matthew MacDell (voice) / Matthew (voice) /

Nick Kroll
Nick Birch / Maury the Hormone Monster / Coach Steve (voice)

John Mulaney
Andrew Glouberman (voice)

Jason Mantzoukas
Jay Bilzerian (voice)

Maya Rudolph
Diane Birch / Constance the Hormone Monstress (voice)

Fred Armisen
Elliot Birch (voice)

Jordan Peele
Ghost of Duke Ellington (voice)

Jenny Slate
Missy Foreman-Greenwald (voice)

Ayo Edebiri
Missy Foreman-Greenwald (voice) / Missy Foreman-Greenwald / Mosaic Missy (voice)

Joe Wengert
Caleb (voice) / Caleb / Lump Humpman (voice) / Caleb / Go-Gurt Thief / Teacher (...

Richard Kind
Marty Glouberman (voice)

Jessica Chaffin
Shannon Glaser (voice) / Shannon Glaser / Grace / Intellect Sphinx (voice) /

Paula Pell
Barbara Glouberman (voice) / (voice) / / Barbara Glouberman / Bubbe (voice) / B...

Neil Casey
Lars (voice) / Detective Dumont / D.N. Ape (voice) / (voice) / Detective Dumont ...

Jak Knight
DeVon (voice) / DeVon / Tall Guy (voice) / DeVon / Gina's Brother #2 (voice)

June Diane Raphael
Devin (voice)

Jon Daly
Judd Birch (voice) / Judd Birch / Pumbaa (voice) / Garrison Keillor / Magician #...

Kat Dennings
Leah Birch (voice) / Leah Birch / Lorraine the Hormone Monstress (voice)

Seth Morris
Greg Glaser (voice) / (voice) / Ninth Grader (voice)

Gina Rodriguez
Gina Alvarez (voice) / Gina Alvarez / Gina Parrot (voice)