Andrew Rannells
Matthew MacDell (voice) / Matthew (voice) /

Nick Kroll
Nick Birch / Maury the Hormone Monster / Coach Steve (voice)

John Mulaney
Andrew Glouberman (voice)

Jason Mantzoukas
Jay Bilzerian (voice)

Maya Rudolph
Diane Birch / Constance the Hormone Monstress (voice)

Fred Armisen
Elliot Birch (voice)

Jordan Peele
Ghost of Duke Ellington (voice)

Jenny Slate
Missy Foreman-Greenwald (voice) /

Joe Wengert
/ Caleb (voice) / Caleb / Lump Humpman (voice) / Steven Tyler Glade Plug-In / Z...

Ayo Edebiri
Missy Foreman-Greenwald (voice) / Missy Foreman-Greenwald / Mosaic Missy (voice)

June Diane Raphael
Devin (voice) /

Richard Kind
Marty Glouberman (voice) /

Jessica Chaffin
Shannon Glaser (voice) / / Shannon Glaser / Grace / Intellect Sphinx (voice)

Neil Casey
Lars (voice) / / Lars / Boy (voice) / Lars / Jew Penis (voice) / Lars / Detecti...

Paula Pell
Barbara Glouberman (voice) / / (voice) / Barbara Glouberman / Lola's Couch Pill...

Jon Daly
Judd Birch (voice) / / Garrison Keillor / Magician #1 (voice) / Judd Birch / Pu...

Jak Knight
DeVon (voice) / DeVon / Tall Guy (voice) / DeVon / Gina's Brother #2 (voice)

Chelsea Peretti
Monica Foreman-Greenwald (voice) / / Cellsea (voice) / Diaphragm (voice)

Seth Morris
Greg Glaser (voice) / / (voice) / Ninth Grader (voice)

Mark Rivers
/ (voice) / Siamese Twin #1 (voice) / Ghost of David Bowie (voice) / Vendor (vo...