Neil deGrasse Tyson
Self - Host

Ann Druyan

Carl Sagan
Self (archive footage) / Self (voice) (archive footage)

Seth MacFarlane
President Harry S. Truman (voice) / (voice) / Giordano Bruno (voice) / Sherwood ...

Stoney Emshwiller
Guard (voice) /

André Sogliuzzo
Christopher Wren (voice) / Weichelberger (voice)

Christopher Emerson
Acolyte (voice) /

Piotr Michael
Edmund Muskie (voice) / Lab Assistant (voice)

Enn Reitel
Henry Norris Russell (voice) /

Keythe Farley
(voice) /

Patrick Stewart
William Herschel (voice)

Elizardo Torrez
Young Carl Sagan

Trang Vo
(voice) / Birthmark Woman

John Steven Rocha
Bellaramine (voice)

Paul Telfer
Angry Scholar #2 (voice)

Jonathan Morgan Heit
John Herschel (voice)

Cary Elwes
Edmond Halley / Robert Hooke (voice)

Tom Konkle
Samuel Pepys (voice)

Alexander Siddig
Isaac Newton (voice)

Alfred Molina
Ibn al-Haytham (voice)