Marvel's Ultimate Spider-Man

Drake Bell
Peter Parker / Spider-Man (voice)

Misty Lee
Aunt May Parker (voice)

J.K. Simmons
J. Jonah Jameson (voice)

Ogie Banks
Luke Cage / Power Man (voice)

Chi McBride
Nick Fury (voice)

Clancy Brown
/ Taskmaster (voice)

Logan Miller
Sam Alexander / Nova (voice)

Kevin Michael Richardson
/ Groot (voice)

Tara Strong
/ Mary Jane (voice)

Matt Lanter
Harry Osborn (voice) / Flash Thompson / Agent Venom / Harry Osborn (voice)

Tyler James Williams
Prowler (voice)

Quinton Flynn

Clark Gregg
Phil Coulson (voice)

Adrian Pasdar

John DiMaggio

Steven Weber

Caitlyn Taylor Love
Ava Ayala / White Tiger (voice)

Tom Kenny
Dr. Otto Octavius / Bentley Wittman (voice)

Fred Tatasciore

Rob Paulsen