Samuel L. Jackson
Afro Samurai / Ninja Ninja (voice)

Phil LaMarr
Brother 1 / Brother 3 / Brother 5 / Kuro / Teen Afro Samurai (voice)

Yuri Lowenthal
Kuma / Jinno (voice)

John DiMaggio
Brother 2 (voice)

Terrence 'T.C.' Carson
Sword Master (voice)

Ron Perlman
Justice (voice)

Fred Tatasciore
Juzo (voice) / Shuzo (voice)

Tara Strong
Jiro (voice) / Otsuru (voice)

Kelly Hu
Okiku (voice)

Grey DeLisle
Oyuki (voice)

James Arnold Taylor
Yashichi (voice)

Jeff Bennett
Foo (voice)

Steve Blum
Assassin (voice)