David Kaye
Optimus Prime (voice)

Bumper Robinson
Bumblebee (voice)

Corey Burton
Ratchet / Megatron (voice)

Bill Fagerbakke
Bulkhead (voice)

Jeff Bennett
Prowl (voice)

Tara Strong
Sari Sumdac (voice)

Tom Kenny
Isaac Sumdac (voice)

Phil LaMarr
Jazz (voice)

Townsend Coleman
Sentinel Prime (voice) / Tracks (voice)

Cree Summer
Blackarachnia (voice)

Susan Blu
Arcee (voice) / Flareup (voice)

Alexander Polinsky
Headmaster (voice)

John Moschitta Jr.
Blurr (voice)

Peter Stormare
Prometheus Black / Meltdown (voice)

Brian Posehn
Nanosec (voice)

Kath Soucie
Professor Princess (voice)

"Weird Al" Yankovic
Wreck-Gar (voice)

Fred Willard
Swindle (voice)

Kevin Michael Richardson
Omega Supreme (voice)

Judd Nelson
Rodimus (voice)