Catherine O'Hara
Mrs. Frankenstein / Weird Girl / Gym Teacher (voice)

Martin Short
Mr. Frankenstein / Mr. Burgemeister / Nassor (voice)

Martin Landau
Mr. Rzykruski (voice)

Charlie Tahan
Victor Frankenstein (voice)

Atticus Shaffer
Edgar 'E' Gore (voice)

Winona Ryder
Elsa Van Helsing (voice)

Robert Capron
Bob (voice)

James Hiroyuki Liao
Toshiaki (voice)

Conchata Ferrell
Bob's Mom (voice)

Tom Kenny
New Holland Townsfolk (voice)

Frank Welker
Sparky (voice) (uncredited)

Dee Bradley Baker
Persephone / Shelly / Were-Rat / Colossus / Mr. Whiskers / Driver (voice) (uncre...

Jeff Bennett
Giant Sea Monkeys (voice) (uncredited)