Johnny Depp
Rango / Lars (voice)

Isla Fisher
Beans (voice)

Ned Beatty
Mayor (voice)

Bill Nighy
Rattlesnake Jake (voice)

Abigail Breslin
Priscilla (voice)

Alfred Molina
Roadkill (voice)

Stephen Root
Doc / Merrimack / Mr. Snuggles (voice)

Harry Dean Stanton
Balthazar (voice)

Timothy Olyphant
Spirit of the West (voice)

Ray Winstone
Bad Bill (voice)

Ian Abercrombie
Ambrose (voice)

Gil Birmingham
Wounded Bird (voice)

Claudia Black
Angelique (voice)

Blake Clark
Buford (voice)

John Cothran
Elgin (voice)

Patrika Darbo
Delilah / Maybelle (voice)

George DelHoyo
Señor Flan - Mariachi Accordion (voice)

Maile Flanagan
Lucky (voice)

Charles Fleischer
Elbows (voice)

Beth Grant
Bonnie (voice)