Henry Fonda
Abraham Lincoln

Alice Brady
Abigail Clay

Marjorie Weaver
Mary Todd

Arleen Whelan
Sarah Clay

Eddie Collins
Efe Turner

Pauline Moore
Ann Rutledge

Richard Cromwell
Matt Clay

Donald Meek
Prosecutor John Felder

Eddie Quillan
Adam Clay

Spencer Charters
Judge Herbert A. Bell

Ward Bond
John Palmer Cass

Jack Kelly
Matt Clay as a Boy (uncredited)

Edwin Maxwell
John T. Stuart (uncredited)

Milburn Stone
Stephen A. Douglas (uncredited)

Ernie Adams
Man with Lynch Mob (uncredited)

Sam Ash
Townsman Dancing at Party (uncredited)

Arthur Aylesworth
New Salem Townsman (uncredited)

Dorris Bowdon
Carrie Sue (uncredited)

Virginia Brissac
Peach Pie Baker (uncredited)

Paul E. Burns
Loafer (uncredited)