Jim J. Bullock
Adam Seymour 'Queer Duck' Duckstein (voice)

Jackie Hoffman
Lola Buzzard (voice)

Kevin Michael Richardson
Stephen Arlo 'Openly' Gator / Additional Voices (voice)

Billy West
Bi-Polar Bear / Additional Voices (voice)

Estelle Harris
Mrs. Duckstein (voice)

Mark Hamill
Hot Dog Vendor (voice)

Tim Curry
Peccary (voice)

Andy Dick
Elizabeth Taylor / Rex ('Regina') (voice)

Chris Cox
Michael Jackson (voice)

Audrey Wasilewski
Rosie O'Donnell (voice)

Maurice LaMarche
Oscar Wildcat / Martin Duckstein / Additional Voices (voice)

Bruce Vilanch
Bruce Vilanch (voice)

Conan O'Brien
Conan O'Brien (voice)

David Duchovny
Tiny Jesus (voice)

Jeff Bennett
Rev. Vandergelding / Additional Voices (voice)

Kevin Chamberlin
Additional Voices (voice)

Barbara Goodson
Elizabeth Taylor (voice)

Howard Hoffman
Additional Voices (voice)

Nick Jameson
Additional Voices (voice)

Tress MacNeille
Additional Voices (voice)