Basil Rathbone
Sherlock Holmes

Nigel Bruce
Dr. John H. Watson

Alan Mowbray
Major Duncan-Bleek

Dennis Hoey
Inspector Lestrade

Renee Godfrey
Vivian Vedder

Frederick Worlock
Prof. William Kilbane

Mary Forbes
Lady Margaret Carstairs

Skelton Knaggs
Sands - Moran's Accomplice

Billy Bevan
Conductor Taking Tickets

Geoffrey Steele
Roland Carstairs

Janet Murdoch
Mrs. Shallcross (uncredited)

Gerald Hamer
Alf Shallcross (uncredited)

Leyland Hodgson
Senior Conductor (uncredited)

Harry Cording
Mock the Undertaker (uncredited)

Charles Knight
Baggage Car Guard (uncredited)

Boyd Davis
"Inspector MacDonald" (uncredited)

Bobby Wissler
Mock Jr. (uncredited)

Gilbert Allen
Dining Car Steward (uncredited)

C. Aubrey Smith
Elderly gentleman on train station (uncredited)